Saliva Collection Instructions

Like any laboratory test, the proper collection of a saliva specimen is vital to an accurate result. It is important for you as a provider to clearly understand the instructions enclosed in each kit so you can educate your patients or clients to ensure the best sample is submitted.

Steps to Successful Saliva Collection and Accurate Test Resutls:

  • Select the best day for collection, and allow a little extra time on that day.
  • Wash hands and rinse mouth with cool water before collecting saliva.
  • Wash your face before bedtime the night before collecting saliva, and do not apply lotions or creams that night or next morning (some may contain interfering substances). Use of routine cosmetics is permitted.
  • Do not brush or floss your teeth or have dental work done 30 minutes prior to collecting saliva. Slight bleeding from these may alter test results.
  • Do not eat at least one hour before collecting saliva.
  • Use of the straws included to “funnel” saliva into each tube is optional.
  • Each tube should be 3/4 full- bubbles or foam are acceptable.

–> FILL ALL FOUR TUBES regardless of how many tests are ordered <–

Time of Day for Saliva Collection: You must collect saliva four times in one day

PURPLE TUBE should be collected about 30 minutes after awakening (even if staying in bed), before breakfast and brushing your teeth.

GREEN TUBE should be collected before lunch.

ORANGE TUBE should be collected before dinner

BLUE TUBE should be collected before bedtime, preferably at least one hour after dinner.

Guidelines for Women

WOMEN WITH MENSTRUAL CYCLES: Collect saliva between days 19-21 of your cycle, counting the first day of your period as day 1.

POST MENOPAUSAL WOMEN OR WOMEN WHO HAVE HAD A HYSTERECTOMY: Collect saliva any day of the month.

WOMEN TAKING HORMONE REPLACEMENT OR BIRTH CONTROL(REGARDLESS OF MENSTRUAL CYCLE)

  • Use topical hormones no closer than 12 hours before collecting first sample, but no longer than 24 hours. Do not apply hormones the day of testing. If you use your topical hormone twice a day, skip both doses on the day of testing.
  • Use sublingual or troche hormones (under tongue) no closer than 36 hours before testing.
  • Oral hormones (pills) and topical patches should be used as prescribed without change.
  • IUD use does not interfere with testing.